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3DHistech ltd scanning microscope 3dhistech pannoramic midi ii digital slide scanner
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Scanning Microscope 3dhistech Pannoramic Midi Ii Digital Slide Scanner, supplied by 3DHistech ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motic Group microscope
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Microscope, supplied by Motic Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motic Group motic easyscan microscope
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Motic Easyscan Microscope, supplied by Motic Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motic Group motic easyscan
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Motic Easyscan, supplied by Motic Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motic Group digital slide scanner
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Digital Slide Scanner, supplied by Motic Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motic Group application system
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Application System, supplied by Motic Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hamamatsu proprietary image files hamamatsu nanozoomer s60
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Proprietary Image Files Hamamatsu Nanozoomer S60, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Canon inc eos 800d camera
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Eos 800d Camera, supplied by Canon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Motic Group algorithms zen black microscopy software zeiss
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Algorithms Zen Black Microscopy Software Zeiss, supplied by Motic Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Laboratory organisms
Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning <t>microscope</t> (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Organisms, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning microscope (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.

Journal: iScience

Article Title: IL-22 and IL-23 regulate the anticryptococcal response during Cryptococcus deuterogattii infection

doi: 10.1016/j.isci.2024.111054

Figure Lengend Snippet: Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning microscope (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.

Article Snippet: The analysis of the slides was performed using light microscopy on a scanning microscope (3DHISTECH Pannoramic MIDI II Digital Slide Scanner for H&E, PSR and MAB and Motic EasyScan Pro for H&E, MT and PAS).

Techniques: Infection, Staining, Light Microscopy, Microscopy