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Image Search Results
Journal: iScience
Article Title: IL-22 and IL-23 regulate the anticryptococcal response during Cryptococcus deuterogattii infection
doi: 10.1016/j.isci.2024.111054
Figure Lengend Snippet: Analysis of fungal size and titanization wild type (WT) ( n = 5), IL-23 −/− ( n = 5), and IL-22 −/− ( n = 5) C57BL/6 mice were intratracheally (i.t.) infected with 10 4 R265 yeast and observed over time. (A–C) Animals had their lungs surgically collected after euthanasia at 18 dpi and taken for Picrosirius Red (PSR) staining. The analysis of the slides was performed using light microscopy on a scanning microscope (3DHISTECH Pannoramic MIDI Digital Slide Scanner). Cell size measurement was performed using the program (3DHISTECH Slide Viewer). Yeasts size was measured by diameter in 3 separated groups: under 5 μm (<5 μm), usual size between (>5 μm and <10 μm), usual titan cells between (>10 μm and <20 μm) and large titan cells above 20 μm (<20 μm); ( n = 400) for all groups. (A) Donut chart with the relative percentage of yeasts by size for each group. (B) Box chart comparing relative diameter size between groups and (C) Heatmap showing diameter diversity between groups. (D) Representative images for each group of titan yeasts and normal yeasts on lung tissue at (16 dpi) using PAS staining and (18 dpi) using PSR staining (×630 of magnification). Photographs were taken using Motic EasyScan equipment for the 16 dpi time point. ( ) yellow arrows indicate titan cells. ∗∗∗∗ p < 0.0001 for comparisons between groups. Representative data of 3 independent experiments.
Article Snippet: The analysis of the slides was performed using light microscopy on a
Techniques: Infection, Staining, Light Microscopy, Microscopy